NEO PanTracer Tissue

Next Generation Sequencing (NGS)
Epic Aura Integration

PanTracer Tissue is a broad, next‑generation sequencing panel for pan‑solid tumor indications. The assay detects single nucleotide variants (SNV), insertions/deletions (InDels), copy number variants (CNV), and RNA fusions and splice variants across a comprehensive gene set, plus microsatellite instability (MSI) and tumor mutational burden (TMB).

NGS (517 total genes: DNA analysis across 517 genes; RNA fusion/splice analysis across 55 of these genes)

Immunotherapy Markers: MSI, TMB
* MSI-high is defined as ≥20% of loci showing instability; microsatellite-stable (MSS) is defined as <20% of loci showing instability.
* TMB-high is defined as ≥10.0 mutations per megabase (mut/Mb); TMB-low is defined as <10.0 mut/Mb

Legacy Test information:
As of May 29, 2025, this test serves as replacement for the following test:
* Neo Comprehensive – Solid Tumor

Specimen Requirements

A block is preferred for testing: >20% tumor and >5 mm2 of tissue surface area for NGS (~500 tumor cells)(additional 100 neoplastic cells for PD-L1). If submitting 5-micron unstained slides, the following number of slides are requested: Samples with >25 mm2 of tissue: 10 unstained slides (2 sections per slide preferred) Samples with 10-24 mm2 of tissue: 20 unstained slides (2 sections per slide preferred) Please submit 1 additional unstained slide for H&E and 3 additional unstained slides if performing PD-L1 testing.

Storage and Transportation

NYS clients please provide date and time of Collection. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

CPT Code(s)*

81459x1. Add 88360x1 for PD-L1 IHC.

Turnaround time

8-10 Days

Level of Service

Global

Integrations

Epic Aura

See all integrations →

New York Approved: Yes

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.

Biomarkers

DNA Sequencing

SNVs + Indels
ABL1	ABL2	ABRAXAS1	ACVR1	ACVR1B	ADGRA2
AKT1	AKT2	AKT3	ALK	ALOX12B	AMER1
ANKRD11	ANKRD26	APC	AR	ARAF	ARFRP1
ARID1A	ARID1B	ARID2	ARID5B	ASXL1	ASXL2
ATM	ATR	ATRX	AURKA	AURKB	AXIN1
AXIN2	AXL	B2M	BAP1	BARD1	BBC3
BCL10	BCL2	BCL2L1	BCL2L11	BCL2L2	BCL6
BCOR	BCORL1	BCR	BIRC3	BLM	BMPR1A
BRAF	BRCA1	BRCA2	BRD4	BRIP1	BTG1
BTK	CALR	CARD11	CASP8	CBFB	CBL
CCN6	CCND1	CCND2	CCND3	CCNE1	CD274
CD276	CD74	CD79A	CD79B	CDC73	CDH1
CDK12	CDK4	CDK6	CDK8	CDKN1A	CDKN1B
CDKN2A	CDKN2B	CDKN2C	CEBPA	CENPA	CHD2
CHD4	CHEK1	CHEK2	CIC	COP1	CREBBP
CRKL	CRLF2	CSF1R	CSF3R	CSNK1A1	CTCF
CTLA4	CTNNA1	CTNNB1	CUL3	CUX1	CXCR4
CYLD	DAXX	DCUN1D1	DDR2	DDX41	DHX15
DICER1	DIS3	DNAJB1	DNMT1	DNMT3A	DNMT3B
DOT1L	E2F3	EED	EGFL7	EGFR	EIF1AX
EIF4A2	EIF4E	ELOC	EML4	EMSY	EP300
EPCAM	EPHA3	EPHA5	EPHA7	EPHB1	ERBB2
ERBB3	ERBB4	ERCC1	ERCC2	ERCC3	ERCC4
ERCC5	ERG	ERRFI1	ESR1	ETS1	ETV1
ETV4	ETV5	ETV6	EWSR1	EZH2	FANCA
FANCC	FANCD2	FANCE	FANCF	FANCG	FANCI
FANCL	FAS	FAT1	FBXW7	FGF1	FGF10
FGF14	FGF19	FGF2	FGF23	FGF3	FGF4
FGF5	FGF6	FGF7	FGF8	FGF9	FGFR1
FGFR2	FGFR3	FGFR4	FH	FLCN	FLI1
FLT1	FLT3	FLT4	FOXA1	FOXL2	FOXO1
FOXP1	FRS2	FUBP1	FYN	GABRA6	GATA1
GATA2	GATA3	GATA4	GATA6	GEN1	GID4
GLI1	GNA11	GNA13	GNAQ	GNAS	GPS2
GREM1	GRIN2A	GRM3	GSK3B	H1-2	H2BC5
H3-3A	H3-3B	H3-4	H3-5	H3C1	H3C10
H3C11	H3C12	H3C13	H3C14	H3C15	H3C2
H3C3	H3C4	H3C6	H3C7	H3C8	HGF
HNF1A	HNRNPK	HOXB13	HRAS	HSD3B1	HSP90AA1
ICOSLG	ID3	IDH1	IDH2	IFNGR1	IGF1
IGF1R	IGF2	IKBKE	IKZF1	IL10	IL7R
INHA	INHBA	INPP4A	INPP4B	INSR	IRF2
IRF4	IRS1	IRS2	JAK1	JAK2	JAK3
JUN	KAT6A	KDM5A	KDM5C	KDM6A	KDR
KEAP1	KEL	KIF5B	KIT	KLF4	KLHL6
KRAS	LAMP1	LATS1	LATS2	LMO1	LRP1B
LYN	LZTR1	MAGI2	MALT1	MAP2K1	MAP2K2
MAP2K4	MAP3K1	MAP3K13	MAP3K14	MAP3K4	MAPK1
MAPK3	MAX	MCL1	MDC1	MDM2	MDM4
MED12	MEF2B	MEN1	MET	MGA	MITF
MLH1	MLL	MLLT3	MPL	MRE11	MSH2
MSH3	MSH6	MST1	MST1R	MTOR	MUTYH
MYB	MYC	MYCL	MYCN	MYD88	MYOD1
NAB2	NBN	NCOA3	NCOR1	NEGR1	NF1
NF2	NFE2L2	NFKBIA	NKX2-1	NKX3-1	NOTCH1
NOTCH2	NOTCH3	NOTCH4	NPM1	NRAS	NRG1
NSD1	NTRK1	NTRK2	NTRK3	NUP93	NUTM1
PAK1	PAK3	PAK5	PALB2	PARP1	PAX3
PAX5	PAX7	PAX8	PBRM1	PDCD1	PDCD1LG2
PDGFRA	PDGFRB	PDK1	PDPK1	PGR	PHF6
PHOX2B	PIK3C2B	PIK3C2G	PIK3C3	PIK3CA	PIK3CB
PIK3CD	PIK3CG	PIK3R1	PIK3R2	PIK3R3	PIM1
PLCG2	PLK2	PMAIP1	PMS1	PMS2	PNRC1
POLD1	POLE	PPARG	PPM1D	PPP2R1A	PPP2R2A
PPP6C	PRDM1	PREX2	PRKAR1A	PRKCI	PRKDC
PRKN	PRSS8	PTCH1	PTEN	PTPN11	PTPRD
PTPRS	PTPRT	QKI	RAB35	RAC1	RAD21
RAD50	RAD51	RAD51B	RAD51C	RAD51D	RAD52
RAD54L	RAF1	RANBP2	RARA	RASA1	RB1
RBM10	RECQL4	REL	RET	RHEB	RHOA
RICTOR	RIT1	RNF43	ROS1	RPS6KA4	RPS6KB1
RPS6KB2	RPTOR	RUNX1	RUNX1T1	RYBP	SDHA
SDHAF2	SDHB	SDHC	SDHD	SETBP1	SETD2
SF3B1	SH2B3	SH2D1A	SHQ1	SLIT2	SLX4
SMAD2	SMAD3	SMAD4	SMARCA4	SMARCB1	SMARCD1
SMC1A	SMC3	SMO	SNCAIP	SOCS1	SOX10
SOX17	SOX2	SOX9	SPEN	SPOP	SPTA1
SRC	SRSF2	STAG1	STAG2	STAT3	STAT4
STAT5A	STAT5B	STK11	STK40	SUFU	SUZ12
SYK	TAF1	TBX3	TCF3	TCF7L2	TENT5C
TERC	TERT	TET1	TET2	TFE3	TFRC
TGFBR1	TGFBR2	TMEM127	TMPRSS2	TNFAIP3	TNFRSF14
TOP1	TOP2A	TP53	TP63	TRAF2	TRAF7
TSC1	TSC2	TSHR	U2AF1	VEGFA	VHL
VTCN1	WT1	XIAP	XPO1	XRCC2	YAP1
YES1	ZBTB2	ZBTB7A	ZFHX3	ZNF217	ZNF703
ZRSR2

CNVs
AKT2	ALK	AR	ATM	BRAF	BRCA1
BRCA2	CCND1	CCND3	CCNE1	CDK4	CDK6
CDK8	CHEK1	CHEK2	EGFR	ERBB2	ERBB3
ERCC1	ERCC2	ESR1	FGF1	FGF10	FGF14
FGF19	FGF2	FGF23	FGF3	FGF4	FGF5
FGF6	FGF7	FGF8	FGF9	FGFR1	FGFR2
FGFR3	FGFR4	JAK2	KIT	KRAS	LAMP1
MDM2	MDM4	MET	MYC	MYCL	MYCN
NRAS	NRG1	PDGFRA	PDGFRB	PIK3CA	PIK3CB
PTEN	RAF1	RET	RICTOR	RPS6KB1	TFRC

Genomic Signatures
MSI	TMB

Fusions
EGFR

RNA Sequencing

Fusions
ABL1	AKT3	ALK	AR	AR-V7	AXL
BCL2	BRAF	BRCA1	BRCA2	CDK4	CSF1R
EML4	ERBB2	ERG	ESR1	ETS1	ETV1
ETV4	ETV5	EWSR1	FGFR1	FGFR2	FGFR3
FGFR4	FLI1	FLT1	FLT3	JAK2	KDR
KIF5B	KIT	MET	MLL	MLLT3	MSH2
MYC	NOTCH1	NOTCH2	NOTCH3	NRG1	NTRK1
NTRK2	NTRK3	PAX3	PAX7	PDGFRA	PDGFRB
PIK3CA	PPARG	RAF1	RET	ROS1	RPS6KB1
TMPRSS2

Splice Variant
AR	AR-V7
EGFR	EGFRvIII
EGFRvIII	MET
MET exon 14 Skipping	MET exon 14 Skipping

Last Updated: June 23, 2026

Get in touch

Our Client Services team is on hand to help. Please call us at 866.776.5907, Option 3.