PanTracer Tissue is a broad, next‑generation sequencing panel for pan‑solid tumor indications. The assay detects single nucleotide variants (SNV), insertions/deletions (InDels), copy number variants (CNV), and RNA fusions and splice variants across a comprehensive gene set, plus microsatellite instability (MSI) and tumor mutational burden (TMB).
NGS (517 total genes: DNA analysis across 517 genes; RNA fusion/splice analysis across 55 of these genes)
Immunotherapy Markers: MSI, TMB
* MSI-high is defined as ≥20% of loci showing instability; microsatellite-stable (MSS) is defined as <20% of loci showing instability.
* TMB-high is defined as ≥10.0 mutations per megabase (mut/Mb); TMB-low is defined as <10.0 mut/Mb
Legacy Test information:
As of May 29, 2025, this test serves as replacement for the following test:
* Neo Comprehensive – Solid Tumor
NEO PanTracer Tissue
- Next Generation Sequencing (NGS)
- Epic Aura Integration
Specimen Requirements
A block is preferred for testing: >20% tumor and >5 mm2 of tissue surface area for NGS (~500 tumor cells)(additional 100 neoplastic cells for PD-L1).
If submitting 5-micron unstained slides, the following number of slides are requested:
Samples with >25 mm2 of tissue: 10 unstained slides (2 sections per slide preferred)
Samples with 10-24 mm2 of tissue: 20 unstained slides (2 sections per slide preferred)
Please submit 1 additional unstained slide for H&E and 3 additional unstained slides if performing PD-L1 testing.
Storage and Transportation
NYS clients please provide date and time of Collection. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
CPT Code(s)*
81459x1. Add 88360x1 for PD-L1 IHC.
Turnaround time
8-10 Days
Level of Service
- Global
Integrations
- Epic Aura
New York Approved: Yes
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Biomarkers
DNA Sequencing
| SNVs + Indels | |||||
|---|---|---|---|---|---|
| ABL1 | ABL2 | ||||
| ABRAXAS1 | ACVR1 | ||||
| ACVR1B | ADGRA2 | ||||
| AKT1 | AKT2 | ||||
| AKT3 | ALK | ||||
| ALOX12B | AMER1 | ||||
| ANKRD11 | ANKRD26 | ||||
| APC | AR | ||||
| ARAF | ARFRP1 | ||||
| ARID1A | ARID1B | ||||
| ARID2 | ARID5B | ||||
| ASXL1 | ASXL2 | ||||
| ATM | ATR | ||||
| ATRX | AURKA | ||||
| AURKB | AXIN1 | ||||
| AXIN2 | AXL | ||||
| B2M | BAP1 | ||||
| BARD1 | BBC3 | ||||
| BCL10 | BCL2 | ||||
| BCL2L1 | BCL2L11 | ||||
| BCL2L2 | BCL6 | ||||
| BCOR | BCORL1 | ||||
| BCR | BIRC3 | ||||
| BLM | BMPR1A | ||||
| BRAF | BRCA1 | ||||
| BRCA2 | BRD4 | ||||
| BRIP1 | BTG1 | ||||
| BTK | CALR | ||||
| CARD11 | CASP8 | ||||
| CBFB | CBL | ||||
| CCN6 | CCND1 | ||||
| CCND2 | CCND3 | ||||
| CCNE1 | CD274 | ||||
| CD276 | CD74 | ||||
| CD79A | CD79B | ||||
| CDC73 | CDH1 | ||||
| CDK12 | CDK4 | ||||
| CDK6 | CDK8 | ||||
| CDKN1A | CDKN1B | ||||
| CDKN2A | CDKN2B | ||||
| CDKN2C | CEBPA | ||||
| CENPA | CHD2 | ||||
| CHD4 | CHEK1 | ||||
| CHEK2 | CIC | ||||
| COP1 | CREBBP | ||||
| CRKL | CRLF2 | ||||
| CSF1R | CSF3R | ||||
| CSNK1A1 | CTCF | ||||
| CTLA4 | CTNNA1 | ||||
| CTNNB1 | CUL3 | ||||
| CUX1 | CXCR4 | ||||
| CYLD | DAXX | ||||
| DCUN1D1 | DDR2 | ||||
| DDX41 | DHX15 | ||||
| DICER1 | DIS3 | ||||
| DNAJB1 | DNMT1 | ||||
| DNMT3A | DNMT3B | ||||
| DOT1L | E2F3 | ||||
| EED | EGFL7 | ||||
| EGFR | EIF1AX | ||||
| EIF4A2 | EIF4E | ||||
| ELOC | EML4 | ||||
| EMSY | EP300 | ||||
| EPCAM | EPHA3 | ||||
| EPHA5 | EPHA7 | ||||
| EPHB1 | ERBB2 | ||||
| ERBB3 | ERBB4 | ||||
| ERCC1 | ERCC2 | ||||
| ERCC3 | ERCC4 | ||||
| ERCC5 | ERG | ||||
| ERRFI1 | ESR1 | ||||
| ETS1 | ETV1 | ||||
| ETV4 | ETV5 | ||||
| ETV6 | EWSR1 | ||||
| EZH2 | FANCA | ||||
| FANCC | FANCD2 | ||||
| FANCE | FANCF | ||||
| FANCG | FANCI | ||||
| FANCL | FAS | ||||
| FAT1 | FBXW7 | ||||
| FGF1 | FGF10 | ||||
| FGF14 | FGF19 | ||||
| FGF2 | FGF23 | ||||
| FGF3 | FGF4 | ||||
| FGF5 | FGF6 | ||||
| FGF7 | FGF8 | ||||
| FGF9 | FGFR1 | ||||
| FGFR2 | FGFR3 | ||||
| FGFR4 | FH | ||||
| FLCN | FLI1 | ||||
| FLT1 | FLT3 | ||||
| FLT4 | FOXA1 | ||||
| FOXL2 | FOXO1 | ||||
| FOXP1 | FRS2 | ||||
| FUBP1 | FYN | ||||
| GABRA6 | GATA1 | ||||
| GATA2 | GATA3 | ||||
| GATA4 | GATA6 | ||||
| GEN1 | GID4 | ||||
| GLI1 | GNA11 | ||||
| GNA13 | GNAQ | ||||
| GNAS | GPS2 | ||||
| GREM1 | GRIN2A | ||||
| GRM3 | GSK3B | ||||
| H1-2 | H2BC5 | ||||
| H3-3A | H3-3B | ||||
| H3-4 | H3-5 | ||||
| H3C1 | H3C10 | ||||
| H3C11 | H3C12 | ||||
| H3C13 | H3C14 | ||||
| H3C15 | H3C2 | ||||
| H3C3 | H3C4 | ||||
| H3C6 | H3C7 | ||||
| H3C8 | HGF | ||||
| HNF1A | HNRNPK | ||||
| HOXB13 | HRAS | ||||
| HSD3B1 | HSP90AA1 | ||||
| ICOSLG | ID3 | ||||
| IDH1 | IDH2 | ||||
| IFNGR1 | IGF1 | ||||
| IGF1R | IGF2 | ||||
| IKBKE | IKZF1 | ||||
| IL10 | IL7R | ||||
| INHA | INHBA | ||||
| INPP4A | INPP4B | ||||
| INSR | IRF2 | ||||
| IRF4 | IRS1 | ||||
| IRS2 | JAK1 | ||||
| JAK2 | JAK3 | ||||
| JUN | KAT6A | ||||
| KDM5A | KDM5C | ||||
| KDM6A | KDR | ||||
| KEAP1 | KEL | ||||
| KIF5B | KIT | ||||
| KLF4 | KLHL6 | ||||
| KRAS | LAMP1 | ||||
| LATS1 | LATS2 | ||||
| LMO1 | LRP1B | ||||
| LYN | LZTR1 | ||||
| MAGI2 | MALT1 | ||||
| MAP2K1 | MAP2K2 | ||||
| MAP2K4 | MAP3K1 | ||||
| MAP3K13 | MAP3K14 | ||||
| MAP3K4 | MAPK1 | ||||
| MAPK3 | MAX | ||||
| MCL1 | MDC1 | ||||
| MDM2 | MDM4 | ||||
| MED12 | MEF2B | ||||
| MEN1 | MET | ||||
| MGA | MITF | ||||
| MLH1 | MLL | ||||
| MLLT3 | MPL | ||||
| MRE11 | MSH2 | ||||
| MSH3 | MSH6 | ||||
| MST1 | MST1R | ||||
| MTOR | MUTYH | ||||
| MYB | MYC | ||||
| MYCL | MYCN | ||||
| MYD88 | MYOD1 | ||||
| NAB2 | NBN | ||||
| NCOA3 | NCOR1 | ||||
| NEGR1 | NF1 | ||||
| NF2 | NFE2L2 | ||||
| NFKBIA | NKX2-1 | ||||
| NKX3-1 | NOTCH1 | ||||
| NOTCH2 | NOTCH3 | ||||
| NOTCH4 | NPM1 | ||||
| NRAS | NRG1 | ||||
| NSD1 | NTRK1 | ||||
| NTRK2 | NTRK3 | ||||
| NUP93 | NUTM1 | ||||
| PAK1 | PAK3 | ||||
| PAK5 | PALB2 | ||||
| PARP1 | PAX3 | ||||
| PAX5 | PAX7 | ||||
| PAX8 | PBRM1 | ||||
| PDCD1 | PDCD1LG2 | ||||
| PDGFRA | PDGFRB | ||||
| PDK1 | PDPK1 | ||||
| PGR | PHF6 | ||||
| PHOX2B | PIK3C2B | ||||
| PIK3C2G | PIK3C3 | ||||
| PIK3CA | PIK3CB | ||||
| PIK3CD | PIK3CG | ||||
| PIK3R1 | PIK3R2 | ||||
| PIK3R3 | PIM1 | ||||
| PLCG2 | PLK2 | ||||
| PMAIP1 | PMS1 | ||||
| PMS2 | PNRC1 | ||||
| POLD1 | POLE | ||||
| PPARG | PPM1D | ||||
| PPP2R1A | PPP2R2A | ||||
| PPP6C | PRDM1 | ||||
| PREX2 | PRKAR1A | ||||
| PRKCI | PRKDC | ||||
| PRKN | PRSS8 | ||||
| PTCH1 | PTEN | ||||
| PTPN11 | PTPRD | ||||
| PTPRS | PTPRT | ||||
| QKI | RAB35 | ||||
| RAC1 | RAD21 | ||||
| RAD50 | RAD51 | ||||
| RAD51B | RAD51C | ||||
| RAD51D | RAD52 | ||||
| RAD54L | RAF1 | ||||
| RANBP2 | RARA | ||||
| RASA1 | RB1 | ||||
| RBM10 | RECQL4 | ||||
| REL | RET | ||||
| RHEB | RHOA | ||||
| RICTOR | RIT1 | ||||
| RNF43 | ROS1 | ||||
| RPS6KA4 | RPS6KB1 | ||||
| RPS6KB2 | RPTOR | ||||
| RUNX1 | RUNX1T1 | ||||
| RYBP | SDHA | ||||
| SDHAF2 | SDHB | ||||
| SDHC | SDHD | ||||
| SETBP1 | SETD2 | ||||
| SF3B1 | SH2B3 | ||||
| SH2D1A | SHQ1 | ||||
| SLIT2 | SLX4 | ||||
| SMAD2 | SMAD3 | ||||
| SMAD4 | SMARCA4 | ||||
| SMARCB1 | SMARCD1 | ||||
| SMC1A | SMC3 | ||||
| SMO | SNCAIP | ||||
| SOCS1 | SOX10 | ||||
| SOX17 | SOX2 | ||||
| SOX9 | SPEN | ||||
| SPOP | SPTA1 | ||||
| SRC | SRSF2 | ||||
| STAG1 | STAG2 | ||||
| STAT3 | STAT4 | ||||
| STAT5A | STAT5B | ||||
| STK11 | STK40 | ||||
| SUFU | SUZ12 | ||||
| SYK | TAF1 | ||||
| TBX3 | TCF3 | ||||
| TCF7L2 | TENT5C | ||||
| TERC | TERT Promoter | ||||
| TET1 | TET2 | ||||
| TFE3 | TFRC | ||||
| TGFBR1 | TGFBR2 | ||||
| TMEM127 | TMPRSS2 | ||||
| TNFAIP3 | TNFRSF14 | ||||
| TOP1 | TOP2A | ||||
| TP53 | TP63 | ||||
| TRAF2 | TRAF7 | ||||
| TSC1 | TSC2 | ||||
| TSHR | U2AF1 | ||||
| VEGFA | VHL | ||||
| VTCN1 | WT1 | ||||
| XIAP | XPO1 | ||||
| XRCC2 | YAP1 | ||||
| YES1 | ZBTB2 | ||||
| ZBTB7A | ZFHX3 | ||||
| ZNF217 | ZNF703 | ||||
| ZRSR2 | |||||
| CNVs | |||||
|---|---|---|---|---|---|
| AKT2 | ALK | AR | ATM | BRAF | BRCA1 |
| BRCA2 | CCND1 | CCND3 | CCNE1 | CDK4 | CDK6 |
| CDK8 | CHEK1 | CHEK2 | EGFR | ERBB2 | ERBB3 |
| ERCC1 | ERCC2 | ESR1 | FGF1 | FGF10 | FGF14 |
| FGF19 | FGF2 | FGF23 | FGF3 | FGF4 | FGF5 |
| FGF6 | FGF7 | FGF8 | FGF9 | FGFR1 | FGFR2 |
| FGFR3 | FGFR4 | JAK2 | KIT | KRAS | LAMP1 |
| MDM2 | MDM4 | MET | MYC | MYCL | MYCN |
| NRAS | NRG1 | PDGFRA | PDGFRB | PIK3CA | PIK3CB |
| PTEN | RAF1 | RET | RICTOR | RPS6KB1 | TFRC |
| Genomic Signatures | |||||
|---|---|---|---|---|---|
| MSI | TMB | ||||
| Fusions | |||||
|---|---|---|---|---|---|
| EGFR | |||||
RNA Sequencing
| Fusions | |||||
|---|---|---|---|---|---|
| ABL1 | AKT3 | ALK | AR | AR-V7 | AXL |
| BCL2 | BRAF | BRCA1 | BRCA2 | CDK4 | CSF1R |
| EML4 | ERBB2 | ERG | ESR1 | ETS1 | ETV1 |
| ETV4 | ETV5 | EWSR1 | FGFR1 | FGFR2 | FGFR3 |
| FGFR4 | FLI1 | FLT1 | FLT3 | JAK2 | KDR |
| KIF5B | KIT | MET | MLL | MLLT3 | MSH2 |
| MYC | NOTCH1 | NOTCH2 | NOTCH3 | NRG1 | NTRK1 |
| NTRK2 | NTRK3 | PAX3 | PAX7 | PDGFRA | PDGFRB |
| PIK3CA | PPARG | RAF1 | RET | ROS1 | RPS6KB1 |
| TMPRSS2 | |||||
| Splice Variant | |||||
|---|---|---|---|---|---|
| AR | AR-V7 | ||||
| EGFR | EGFRvIII | ||||
| EGFRvIII | MET | ||||
| MET exon 14 Skipping | MET exon 14 Skipping | ||||
Last Updated: August 04, 2026