NeoTYPE® Esophageal Tumor Profile

The NeoTYPE Esophageal Tumor Profile analyzes 38 biomarkers through a combination of next-generation sequencing (NGS), FISH, and IHC as listed below. Test orders include summary interpretation of all results to help guide treatment decisions. If Pan-TRK IHC is expressed or equivocal, reflex to either NTRK NGS Fusion Panel (Default) or NTRK 1-3 FISH Panel will be added. A microsatellite instability (MSI) NGS result of "indeterminate" will create a reflex to MSI by PCR as long as the tumor percentage is ≥40% and paired normal tissue is available. NGS (32 genes + 2 biomarkers): AKT1, ALK, APC, ARID1A, BLM, BRAF, BRCA1, BRCA2, CDH1, CDKN2A, CTNNB1, ERBB2, ERBB4, FGFR1, FGFR2, FGFR3, HRAS, KIT, KRAS, MET, Microsatellite Instability (MSI), NFE2L2, NOTCH1, NRAS, PDGFRA, PIK3CA, PTEN, SMAD4, SMARCA4, SMO, SRC, TERT Promoter, TP53, Tumor Mutation Burden (TMB) FISH (2 FISH): MET, PTEN (tech-only available) IHC (2 biomarkers): PD-L1 22C3, Pan-TRK (tech-only available for PD-L1).