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MPL Mutation Analysis by NGS
- NGS
Next-generation sequencing of exon 10 of the MPL gene to detect all possible mutations, including those at the W515 and S505 codons. This test may be ordered separately (if JAK2 analysis, specifically JAK2 V617F, is already performed), as a reflex from JAK2 V617F Mutation Analysis, or as part of the MPN JAK2 V617F with Sequential Reflex to JAK2 Exon 12-15, CALR, and MPL. Note: This assay replaces the former MPL Mutation Analysis performed by bi-directional sequencing. NY samples are still processed by bi-directional sequencing until further notice.
Specimen Requirements
Bone Marrow Aspirate: 1-2 mL in EDTA tube. Sodium Heparin is acceptable. Peripheral Blood: 3-5 mL in EDTA tube. Sodium Heparin is acceptable. Note: Test is DNA-based. Please select Extract & Hold - DNA if specimen hold service is desired.
Storage and Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: refrigerate before shipping and ship same day as drawn whenever possible. Do not freeze.
CPT Code(s)*
81339
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Last Updated: October 13, 2025