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Plasma Cell Myeloma IgH Complex FISH Panel
- FISH
This panel includes probes for the detection of specific translocations commonly associated with plasma cell myeloma and multiple myeloma:
FGFR3/IgH t(4;14)
CCND1/IgH t(11;14)
IgH/MAF t(14;16)
IgH/MAFB t(14;20)
Test Customization: Probes for may be ordered separately.
Clinical Significance
The panel is performed when IgH is rearranged. This panel is used to identify the IgH partner gene in myeloma, which has prognostic impact. IgH rearrangements are found in 55-70% of myelomas. Together t(4;14), t(11;14), t(14;16) and t(14;20) are found in approximately 35% of myelomas and in 90% of myelomas when there is an IgH gene rearrangement present.
Specimen Requirements
Bone Marrow Aspirate: 1-2 mL in sodium heparin tube. EDTA tube is acceptable.
Peripheral Blood: 2-5 mL in sodium heparin tube. EDTA tube is acceptable.
Fresh, Unfixed Tissue: Tissue in RPMI.
Bone Marrow/Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: Minimum 7 slides, labeled with specimen type.
Fluids: Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).
Fixed Cell Suspension: A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.
Note for plasma cell enrichment: Will be performed on bone marrow samples unless otherwise directed by the client. Plasma cell enrichment was not validated using peripheral blood; therefore, it is not recommended as a screening specimen unless increased plasma cells are seen on blood smear. Specimens should be received in our laboratory within 72 hours of collection.
Storage and Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. Specimens should be received in our laboratory within 72 hours of collection. NYS clients please provide date and time of Collection.
CPT Code(s)*
88374x4 automated or 88377x4 manual
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Last Updated: September 17, 2025