This panel is designed to detect key genetic abnormalities associated with non-Hodgkin lymphoma (NHL). It includes the following probes:
ALK (2p23)
BCL6 (3q27)
MYC (8q24)
CCND1/IgH t(11;14)
IgH (14q32)
IgH/BCL2 t(14;18)
MALT1 (18q21)
Test Customization: Probes may be ordered separately.
Non-Hodgkin's Lymphoma (NHL) FISH Panel
- FISH
Specimen Requirements
Bone Marrow Aspirate: 1-2 mL in sodium heparin tube. EDTA tube is acceptable.
Peripheral Blood: 2-5 mL in sodium heparin tube. EDTA tube is acceptable.
Fresh, Unfixed Tissue: Tissue in RPMI.
Bone Marrow/Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: Minimum 7 slides, labeled with specimen type.
Fluids: Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).
Fixed Cell Suspension: A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.
H&E slide (required) plus paraffin block.
Cut Slides: H&E slide (required) plus 9 unstained slides cut at 4-5 microns.
Cut Slides: H&E slide (required) plus 3 unstained slides cut at 4-5 microns for single abnormalities.
Note for Tech Only clients: Please circle H&E slide.
Storage and Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: refrigerate before shipping and ship same day as drawn whenever possible. Do not freeze. NYS clients please provide date and time of collection.
CPT Code(s)*
88374x7 automated. Codes may differ if manual analysis is performed.
Turnaround time
3-5 Days
Level of Service
- Global
- Tech Only
Integrations
Request electronic integrations →
New York Approved: Yes
Clinical Significance
The NHL FISH panel is used for the detection of chromosome aberrations observed in lymphoma.
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Biomarkers
Fluorescence In Situ Hybridization (FISH)
| Translocation(s) | |||||
|---|---|---|---|---|---|
| BCL2/IgH t(14;18) | CCND1(BCL1)/IgH t(11;14) | ||||
| IgH (14q32) | MALT1 (18q21) | ||||
| Amplification(s) | |||||
|---|---|---|---|---|---|
| MYC | |||||
| Rearrangement(s) | |||||
|---|---|---|---|---|---|
| ALK | BCL6 | ||||
Last Updated: March 06, 2026