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MPN FISH Panel
- FISH
This FISH Panel is designed to detect key genetic abnormalities associated with myeloproliferative neoplasms. It includes the following probes:
PDGFRa, CHIC2, FIP1L1 (4q12)
PDGFRb (5q33)
FGFR1 (8p11)
BCR/ABL1/ASS1 t(9;22)
Test Customization: Probes may be ordered separately.
Clinical Significance
The MPN FISH panel is indicated in the diagnosis of myeloproliferative neoplasms with eosinophilia and as a guide to therapeutic selection. Patients with BCR-ABL, PDGFRA or PDGFRB rearrangements are responsive to tyrosine kinase inhibitors. There currently is no established TKI therapy for diseases with FGFR1 rearrangement, but some promising new therapies have been reported. If these FISH studies are negative, that does not exclude a myeloproliferative neoplasm and molecular studies for JAK2 and/or MPL mutations should also be considered in the work-up of a possible myeloproliferative neoplasm.
Specimen Requirements
Bone Marrow Aspirate: 1-2 mL in sodium heparin tube. EDTA tube is acceptable.
Peripheral Blood: 2-5 mL in sodium heparin tube. EDTA tube is acceptable.
Fresh, Unfixed Tissue: Tissue in RPMI.
Bone Marrow/Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: Minimum 4 slides, labeled with specimen type.
Fluids: Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).
Fixed Cell Suspension: A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.
Storage and Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: refrigerate before shipping and ship same day as drawn whenever possible. Do not freeze. NYS clients please provide date and time of Collection.
CPT Code(s)*
88374x4 automated. Codes may differ if manual analysis is performed.
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Last Updated: August 27, 2025