Indication: early-stage Breast Cancer
Intended Use:
The MAF (16q23) / D16Z3 FISH probe (MAFTEST®) is intended for use in a non-automated semi-qualitative fluorescence in situ hybridization assay to detect amplification involving the MAF gene region at 16q23 in a dual color assay on formalin fixed paraffin embedded breast cancer tissue sections. The centromere 16 specific centromere repeat probe (D16Z3) is included as control on hybridization efficiency.
Clinical Utility:
• MAF gene amplification in primary tumors has been associated with an increased risk of relapse, death, and metastasis, especially bone metastasis, in early-stage breast cancer patients.
• MAF gene amplification may be considered as an adverse prognostic factor of the disease.
MAF Amplification for Breast Cancer
- FISH
Specimen Requirements
Paraffin block
If submitting slides (instead of a block):
* H&E Slide: One (1) unbaked, unstained slide for H&E staining (required) or client H&E.
* Cut Slides: Two (2) unstained slides cut at 4-5 microns.
Specimen identification:
Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage and Transportation
Slides may be stored at room temperature; transport with cold pack, making sure cold pack is not in direct contact with specimen.
NYS clients: Provide date and time of collection.
CPT Code(s)*
88377
Turnaround time
3-5 Days
Level of Service
- Global
- Tech Only
Integrations
Request electronic integrations →
New York Approved: No
Clinical Significance
Clinical Utility:
• MAF gene amplification in primary tumors has been associated with an increased risk of relapse, death, and metastasis, especially bone metastasis, in early-stage breast cancer patients.
• MAF gene amplification may be considered as an adverse prognostic factor of the disease.
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Biomarkers
Fluorescence In Situ Hybridization (FISH)
| Amplification(s) | |||||
|---|---|---|---|---|---|
| MAF | |||||
Last Updated: November 12, 2026