Low-Grade/Small B-Cell Lymphoma FISH Panel

This targeted panel is appropriate when clinical and morphologic evaluation is most suggestive of a low-grade/small B-cell lymphoma, and high-grade lymphomas (such as large cell anaplastic and Burkitt lymphoma) are not a diagnostic consideration. BCL6 evaluation (included in this panel) can provide further prognostic information in cases of follicular lymphoma since it can indicate a higher risk for transformation to aggressive lymphoma.

Low-grade/small B-cell lymphomas include follicular lymphoma, mantle cell lymphoma (MCL), and marginal zone lymphoma (MZL)/MALT lymphomas. Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) is also within this category, but typically has a peripheral blood lymphocytosis and is better evaluated by the CLL FISH Panel or NeoTYPE CLL Profile.

Bone Marrow Aspirate: 1-2 mL in sodium heparin tube. EDTA tube is acceptable.
Peripheral Blood: 2-5 mL in sodium heparin tube. EDTA tube is acceptable.
Fresh, Unfixed Tissue: Tissue in RPMI.
Bone Marrow/Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: Minimum 4 slides, labeled with specimen type.
Fluids: Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).
Fixed Cell Suspension: A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.
Cut Slides: H&E slide (required) plus 6 unstained slides cut at 4-5 microns.
Cut Slides: H&E slide (required) plus 3 unstained slides cut at 4-5 microns for single abnormalities.
Note for Tech Only clients: Please circle H&E slide.