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High-Grade B-Cell Lymphoma Reflex FISH Panel
- FISH
This panel is designed to detect key genetic abnormalities associated with B-cell lymphoma, including double-hit (DHL) and triple-hit lymphoma (THL). It includes the following probes:
MYC (8q24)
MYC/IgH/CEN8 t(8;14)
Reflex Scheme:
(1) If MYC/IgH/CEN8 t(8;14) is positive, reflex to test for BCL2 (18q21) and BCL6 (3q27).(2) If MYC (8q24) is positive but MYC/IgH/CEN8 t(8;14) is negative, reflex to test for BCL2 (18q21), BCL6 (3q27), IGK/MYC t(2;8), IGL/MYC t(8;22), and BCL6/MYC t(3;8).
Test Customization: Tech Only clients may order probes individually.
Specimen Requirements
Bone Marrow Aspirate: 1-2 mL in sodium heparin tube. EDTA tube is acceptable.
Peripheral Blood: 2-5 mL in sodium heparin tube. EDTA tube is acceptable.
Fresh, Unfixed Tissue: Tissue in RPMI. Bone Marrow/Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: Minimum 3 slides, labeled with specimen type.
Fluids: Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).
Fixed Cell Suspension: A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.
H&E slide (required) plus paraffin block.
Cut Slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns.
Storage and Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: refrigerate before shipping and ship same day as drawn whenever possible. Do not freeze. NYS clients please provide date and time of Collection.
CPT Code(s)*
88374x2 automated or 88377x2 manual without reflex; with reflex option 1, add 88374x2 automated or 88377x2 manual; with reflex option 2, add 88374x5 automated or 88377x5 manual
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Last Updated: August 05, 2025