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High-Grade/Large B-Cell Lymphoma Panel
- FISH
This panel differentiates double-hit or triple-hit lymphomas (which have MYC rearrangements together with BCL2 and/or BCL6 rearrangements) from Burkitt lymphoma or diffuse large B-cell lymphoma. It includes the following probes:
MYC (8q24)
MYC/IgH/CEN8 t(8;14)
BCL6 (3q27)
BCL2 (18q21)
Optional Reflex:
(1) If MYC (8q24) is positive and MYC/IgH/CEN8 t(8;14) is negative reflex to test for IGK/MYC t(2;8), IGL/MYC t(8;22), and BCL6/MYC t(3;8).
Test Customization: Tech Only clients may order probes individually.
Specimen Requirements
Bone marrow aspirate: 1-2 mL sodium heparin tube. EDTA tube is acceptable.
Peripheral blood: 2-5 mL sodium heparin tube. EDTA tube is acceptable.
Fresh, unfixed tissue: Tissue in RPMI.
Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: Minimum 3 slides labeled with specimen type. Three slides are required for the base 3-probe panel, plus 1 slide each if optional 14;18 or 8;14 probes are added.
Fluids:Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).
Fixed Cell Suspension: A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.
H&E slide (required) plus paraffin block.
Cut Slides: H&E slide (required) plus 8 unstained slides cut at 4-5 microns.
Storage and Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. NYS clients please provide date and time of Collection.
CPT Code(s)*
88374x4 automated. If reflex is added, add 88374x3. Codes may differ if manual analysis is performed.
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Biomarkers
Fluorescence In Situ Hybridization (FISH)
| Amplification(s) | |||||
|---|---|---|---|---|---|
| MYC | |||||
| Rearrangement(s) | |||||
|---|---|---|---|---|---|
| BCL2 | BCL6 | MYC-IgH | |||
Last Updated: March 09, 2026