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Anaplastic Large Cell Lymphoma FISH Panel
- FISH
This panel is designed to detect key genetic abnormalities associated with anaplastic large cell lymphoma (ALCL) and peripheral T-cell lymphoma (PTCL). It includes the following probes:
ALK (2p23)
TP63 (3q28)
TBL1XR1/TP63 [inv(3)(q26q28)]
DUSP22-IRF4 (6p25.3)
Testing Scheme: TP63 (3q28) and TBL1XR1/TP63 [inv(3)(q26q28)] are performed together and reported as co-dependent for the result and interpretation of TP63 rearrangement status.
Test Customization: Tech Only clients may order probes individually.
Clinical Significance
The ALCL FISH Panel is used for the detection of recurrent chromosome abnormalities observed in patients with anaplastic large cell lymphoma (ALCL) which may classify those patients into specific risk groups. This panel includes ALK (2p23) rearrangement testing. Patients with ALK-positive ALCL have a favorable prognosis compared to ALK-negative ALCL patients. This panel also includes two assays to detect TP63 rearrangements. Rearrangements of the TP63 gene encoding p63 fusion defines a subset of ALK-negative ALCL cases and are associated with aggressive course and poor outcome as compared to peripheral T-cell lymphoma without these rearrangements.
The TBL1XR1/TP63 fusion [inv(3)] has also been reported in rare cases of peripheral T-cell lymphoma NOS, mycosis fungoides, diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma. This panel also includes the DUSP22-IRF4 rearrangement test which has been reported in CD30-positive, ALK-negative ALCL and is associated with favorable clinical outcome. DUSP22-IRF4 rearrangements have been also reported in patients with lymphomatoid papulosis (LyP).
Specimen Requirements
H&E slide (required) plus paraffin block.
Cut Slides: H&E slide (required) plus 6 unstained slides cut at 4-5 microns.
Note for Tech Only clients: Please circle H&E slide.
Storage and Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. NYS clients please provide date and time of Collection.
CPT Code(s)*
88374x4 automated or 88377x4 manual
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Last Updated: August 27, 2025