NeoGenomics performs tests using a number of methodologies and technologies. Below you will find more information about the types of test available. You can find out more about each testing methodology by clicking on the links below.
Cytogenetics is the specialized area of laboratory medicine involving the study of normal and abnormal chromosomes and their relationship to disease. Cytogenetic studies are often ordered to answer diagnostic, prognostic, and predictive questions in the treatment of hematologic malignancies and solid tumors.
Fluorescence In-Situ Hybridization
Fluorescence in-situ hybridization (FISH) relies on attachment of specific DNA sequences to chromosomes to reveal very subtle chromosomal abnormalities or to efficiently scan large numbers of cells. FISH helps bridge abnormality detection between the chromosomal and DNA sequence levels. It is also called molecular cytogenetics.
Flow Cytometry is a rapid way to measure the characteristics of individual cells. Hematopoietic cells (blood, bone marrow aspirate, lymph nodes, etc.) are labeled with selective fluorescent antibodies and quantified according to their surface antigens. These fluorescent antibodies bind to specific cells in a pattern of antigen expression that is used to identify particular malignant cell types.
Immunohistochemistry or IHC is the process of localizing proteins in a tissue section through binding of labeled antibodies to specific antigens. Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors.
Molecular genetics is a set of techniques to analyze the structure of genes at the DNA sequence level, or to assess a gene’s activity at the RNA level. PCR, RT-PCR, Sanger sequencing, next-gen sequencing (NGS), and other methods are used to detect mutations and their effects in the qualitative and quantitative assays offered at NeoGenomics.
NeoTYPE™ Cancer Profiles
NeoTYPE™ Cancer Profiles consists of 23 different tumor-specific hematologic and solid tumor profiles. These profiles are multi-method test panels based on next-gen sequencing that identify the genetic changes most significant for confirming diagnosis, disease classification, assessing prognosis, predicting response to therapy and identifying clinical trial opportunities.