Fluorescence in-situ Hybridization (FISH) Tests

• Disease(s):  Acute myeloid leukemia
• Probes: 
• 5q-, -5  (5p15, 5q31, 5q33)
• 7q-, -7  (7q31, Cen 7) 
• Trisomy 8  (Cen 8) 
• MLL (11q23)
• 20q- (20q12, 20qter)
• RUNX1/RUNX1T1 (ETO/AML1) t(8;21) 
• PML/RARA t(15;17) 
• CBFB inv(16), t(16;16)
• Probes may be ordered separately except +8 and 20q- which are combined.  
  

• Disease(s): Bladder cancer
• Probes:
• +3 (Cen 3)
• +7 (Cen 7)
• p16 (9p21)
• +17 (Cen 17)

• Disease(s): CLL
• Probes:
• ATM (11q-)
• Trisomy 12 (Cen 12)
• 13q- (13q14, 13q34)
• CCND1/IgH t(11;14)
• p53 (17p-)

• Disease(s): Breast cancer
• Probes:
• HER2 (17q11.2-q12)
• 17 (Cen 17)

• Disease(s): Myelodysplastic syndrome
• Probes: 5q-, -5 (5p15, 5q31, 5q33)
• 7q-, -7 (7q31, Cen 7)
• Trisomy 8 (Cen 8)
• MLL (11q23)
• 20q- (20q12, 20qter)
• Probes may be ordered separately except +8 and 20q- which are combined.

• Disease(s):  Myeloproliferative neoplasms
• Probes:
• PDGFRa, CHIC2, FIP1L1 (4q12)
• PDGFRb (5q33)
• FGFR1 (8p11)
• BCR/ABL1 t(9;22) including ASS1 (9q34)
• Probes may be ordered separately.
  

• Disease(s): Multiple myeloma
• Probes:
• FGFR3/IgH t(4;14)
• 13q-, -13 (13q14, 13q34)
• IgH/MAF t(14;16)
• p53 (17p-)

• Disease(s): Barrett's esophagus, esophageal adenocarcinoma
• Probes:
• MYC (8q24)
• p16 (CDKN2A at 9p21)
• HER2 (ERBB2 at 17q12)
• ZNF217 (20q13)

This 4-probe FISH assay is performed on cytology brushings from Barrett esophagus to determine patterns of chromosomal gain and loss suggestive of esophageal adenocarcinoma/high-grade dysplasia vs. low grade dyplasia/non-dysplastic tissue. Sensitivity is 86% and specificity is 67% for differentiating the high-risk from the low-risk groups. Sensitivity and specificity are higher when only nodules rather than the entire esophagus are brushed. Cells from nodular surfaces will be tested first (if available) and reported as a final result if positive, or testing will reflex to the pan brushing sample if negative.

• Disease(s): NHL
• Probes:
• ALK (2p23)
• BCL6 (3q27)
• MYC (8q24)
• CCND1/IgH t(11;14)
• IgH (14q32)
• IgH/BCL2 t(14;18)
• MALT1 (18q21)

• Disease(s): Anaplastic large cell lymphoma, NHL
• Probes: ALK (2p23)

• Disease(s): Diffuse large B-cell lymphoma, NHL
• Probes: BCL6 (3q27)

• Disease(s): AML, AMML (AML-M4Eo)
• Probes: CBFB inv(16), t(16;16)

• Disease(s): Multiple myeloma, MGUS
• Probes: FGFR3/IgH t(4;14)

• Disease(s): Follicular lymphoma, NHL
• Probes: IgH/BCL2 t(14;18)

• Disease(s): Myeloma, MGUS
• Probes: IgH/MAFB t(14;20)

This probe combination may be ordered separately or added to any of our myeloma FISH panels.

• Disease(s): ALL, AML
• Probes: MLL (11q23)

• Disease(s): Burkitt lymphoma, NHL
• Probes:
• Trisomy 8 (Cen 8)
• MYC/IgH t(8;14)

• Disease(s): Chronic eosinophilic leukemia, MPN
• Probes: PDGFRA, CHIC2, FIP1L1 (4q12)

• Disease(s): Prostate cancer, melanoma, squamous cell carcinoma of the head and neck (PTEN deletions)
• Probes:
• PTEN (10q23)
• Centromere 10

This test may be ordered separately or as part of the NeoTYPE Solid Tumor Profiles (Breast, Colorectal, Gastric, Lung, Other).

• Disease(s): AML-M2
• Probes: RUNX1T1/RUNX1 (ETO/AML1) t(8;21)