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Methodology |
Molecular |
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Test Name |
ABL1 Kinase Domain Mutation Analysis |
|
Test Description |
RT-PCR and sequencing of the BCR-ABL1 fusion transcript for qualitative detection of mutations associated with imatinib resistance |
|
Clinical Significance |
Testing is recommended in CML with poor initial response to imatinib, relapse, or progression to accelerated/blast phase. Presence and identity of mutation may direct management to alternative drugs or stem cell transplant. |
|
Specimen Requirements |
- Peripheral blood: 5 mL in EDTA tube.
- Bone marrow: 2 mL in EDTA tube.
|
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Storage and Transportation |
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. Ship same day as drawn whenever possible; specimens <72 hours old preferred. |
|
Turn around Time |
10 days |
|
CPT Codes |
AMA 2013: 81401 |